Sunday, September 7, 2008

Afternoon

Arrival

Registration 7:00 pm

Putah Creek Lodge (at the bottom of the map below)

Ben Hankamer

Thomas Walz

Henning Stahlberg

Bryant Gipson

Po-Lin Chiu

Ludovic Renault

7:00 pm

Dinner Buffet. Putah Creek Lodge (at the bottom of the map below)

Monday, September 8, 2008

8:00 am

Breakfast.

9:00 am

Welcome

Bryant Gipson

Tom Walz

Henning Stahlberg

9:30 am

SESSION CHAIR: Daniel Levy

Detergents and their properties

Michael Landsberg

10.10 am

Lipids and their properties

Catherine Venien-Bryan

10:50 am

Coffee Break

11.15 am

SESSION CHAIR: Daniel Levy

2D crystallization: Dialysis

Thomas Walz

12:00 pm

Lunch. Segundo Dining Area

1:00 pm

SESSION CHAIR: Tom Walz

2D crystallization: Monolayer approach & biobeads

Daniel Levy

1.30 pm

2D crystallization: Detergent chelation and dilution

Herve Remigy

2:00 pm

Practical: 2D crystallization

-      Monolayer Trial
(Daniel Levy)

-      Dialysis Trial
(Tom Walz, Po-Lin)

-      Biobead Trial
(Ben Hankamer, Ludovic Renault)

-      Dilution Demo
(Remigy / Engel)

Practical: Sample Preparation / CryoEM data collection

-      Cryo-Neg. stain (Ludovic Renault)

-      Cryo: Back injection (H-T Chou, Yifan Cheng)

-      Cryo: Sandwich (Hui-Ting, Nobuhiko Gyobu)

-      Cryo-EM imaging (Dieter, Po-Lin)

-      e-Diffraction (Yifan Cheng, James Buban)

Practical: Image Processing

-      Installation of MRC, 2dx, IPLT on private portable computers

-      2D processing with MRC (Anchi Cheng)

-      2D processing with 2dx_image (Bryant Gipson)

Anchi Cheng

Andreas Schenk

Ansgar Philippsen

Bryant Gipson

Daniel Levy

Dieter Typke

Nobuhiko Gyobu

Herve Remigy

Hui-Ting Chou

James Buban

Ludovic Renault

Po-Lin Chiu

Tom Walz

Yifan Cheng

5:30 pm

Dinner, Segundo Dining Area.

6:30 pm

Student Posters and Coffee, in 1022 LSA

7:30 pm

SESSION CHAIR: Tom Walz & Daniel Levy

Round table discussion: Improved approaches to 2D crystal production

8.15 pm

Posters/open hour

Tuesday, September 9, 2008

8:00 am

Breakfast.

9:00 am

SESSION CHAIR: Henning Stahlberg

TEM: Instrumentation for high resolution data collection

-      Vacuum, Gun (FEG/Thermal, Acc. Voltage), Stage (LN2, Helium), Low-Dose, Spot Scanning

Bob Glaeser

9:45 am

Sample Preparation

-      Methods for enriching crystals from a mixture of crystals and vesicles.

-      Harvesting crystals: preventing damage to crystals during transfer

-      Benefits of glucose, trehalose etc.

-      Back injection, Carbon sandwich and other techniques

Nobuhiko Gyobu

10:30 am

Coffee Break

10:45 am

SESSION CHAIR: Bob Glaeser

Data collection: Imaging

-      Alignment (Coherence, Beam tilt, stability)

-      Data collection (Defocus, Astigmatism)

-      Dose limitation and dose measurement

Dieter Typke

11:20 pm

Data collection: Diffraction

-      Principle, Alignment, Operation mode (Exp. Time, etc.), Film/CCD

Yifan Cheng

12:00 pm

Lunch. Segundo Dining Area

1:00 pm

SESSION CHAIR: Ben Hankamer

TEM and other developments at FEI

TBD

1:20 pm

Hard/Software advances for high-resolution electron crystallography (JEOL)

Barbara Armbruster

1:40 pm

CCD camera and other developments at TVIPS

Matthias Stumpf

2:00 pm

to 2:20 pm

High performance cameras for electron crystallography (GATAN)

Chris Booth

2:30 pm

Practical: 2D crystallization

-      Monolayer Trial
(Daniel Levy)

-      Dialysis Trial
(Tom Walz, Po-Lin)

-      Biobead Trial
(Ben Hankamer, Ludovic Renault)

-      Dilution Demo
(Remigy / Engel)

Practical: Sample Preparation / CryoEM data collection

-      Cryo-Neg. stain (Ludovic Renault)

-      Cryo: Back injection (H-T Chou, Yifan Cheng)

-      Cryo: Sandwich (Hui-Ting, Nobuhiko Gyobu)

-      Cryo-EM imaging (Dieter, Po-Lin)

-      e-Diffraction (Yifan Cheng, James Buban)

Practical: Image Processing

-      Installation of MRC, 2dx, IPLT on private portable computers

-      2D processing with MRC (Anchi Cheng)

-      2D processing with 2dx_image (Bryant Gipson)

Anchi Cheng

Andreas Schenk

Ansgar Philippsen

Bryant Gipson

Daniel Levy

Dieter Typke

Nobuhiko Gyobu

Herve Remigy

Hui-Ting Chou

James Buban

Ludovic Renault

Po-Lin Chiu

Tom Walz

Yifan Cheng

5:30 pm

Dinner, Segundo Dining Area

6:30 pm

Student Posters and Coffee, in 1022 LSA

7:30 pm

SESSION CHAIR: Henning Stahlberg

Contrast formation in the TEM: Phase Shifting devices, aberration vs. aberration correction.

Bob Glaeser

8:15 pm

Electron detection: DQE, MTF, Scanner vs. Film, CMOS, deceleration CCD

Ken Downing

9.00 pm

Posters/open hour

Wednesday, September 10, 2008

8:00 am

Breakfast.

9:00 am

SESSION CHAIR: Ben Hankamer

Fourier Theory: 2D

-      Real space / Fourier space

-      FFT, Correlation, Convolution, Autocorrelation

-      Lattice lines

Bob Glaeser

9:40 am

Image Processing in 2D: MRC software for real-space images

-      Format, Documentation, Work-flow

-      Programs, Unbending

Anchi Cheng

10:25 am

Coffee Break

10:40 am

SESSION CHAIR: Henning Stahlberg

2dx software: Introduction

Bryant Gipson

11:30 am

IPLT software: Introduction

Ansgar Philippsen

12:00 pm

Lunch. Segundo Dining Area

1:00 pm

Practical: 2D crystallization

-      Monolayer Trial
(Daniel Levy)

-      Dialysis Trial
(Tom Walz, Po-Lin)

-      Biobead Trial
(Ben Hankamer, Ludovic Renault)

-      Dilution Demo
(Remigy / Engel)

Practical: Sample Preparation / CryoEM data collection

-      Cryo-Neg. stain (Ludovic Renault)

-      Cryo: Back injection (H-T Chou, Yifan Cheng)

-      Cryo: Sandwich (Hui-Ting, Nobuhiko Gyobu)

-      Cryo-EM imaging (Dieter, Po-Lin)

-      e-Diffraction (Yifan Cheng, James Buban)

Practical: Image Processing

-      Installation of MRC, 2dx, IPLT on private portable computers

-      2D processing with MRC (Anchi Cheng)

-      2D processing with 2dx_image (Bryant Gipson)

Anchi Cheng

Andreas Schenk

Ansgar Philippsen

Bryant Gipson

Daniel Levy

Dieter Typke

Nobuhiko Gyobu

Herve Remigy

Hui-Ting Chou

James Buban

Ludovic Renault

Po-Lin Chiu

Tom Walz

Yifan Cheng

4:00 pm

SESSION CHAIR: Tom Walz

Beam-induced resolution loss

-      SpotScanning

-      Beam-induced drum-head movement vs. charging

Bob Glaeser

4:30 pm

2D crystal screening

Iban Ubarretxena-Belandia

4:50 pm

Basel System for 2D crystal screening

Herve Remigy

5:10 pm

Discussion: How do we address the crystallization bottleneck

Tom Walz

5:30 pm

Dinner, Segundo Dining Area

7:00 pm

SESSION CHAIR: Iban Ubarretxena-Belandia

Molecular mechanisms of membrane transport studied by cryo-EM

Werner Kühlbrandt

7:45 pm

Membr. assoc. proteins in eicosanoid and glutathione metabolism (MAPEGs)

Hans Hebert

8:30 pm

Open hour

Thursday, September 11, 2008

8:00 am

Breakfast.

9:00 am

SESSION CHAIR: Andreas Schenk

Symmetry

-      Point groups, Unit cells and lattices, Symmetry operations

-      Space groups, plane groups, crystallographic notation

Michael Landsberg

9:30 am

Contrast transfer function (CTF)

Henning Stahlberg

9:45 am

Tilt geometry

Henning Stahlberg

10:00 am

A Maximum Likelihood approach to 2D crystals

Henning Stahlberg

10:15 am

Unbending: Effect of unbending before vs. after CTF correction

Andreas Schenk

10:45 am

Coffee Break

11:00 am

SESSION CHAIR: Andreas Engel

IPLT software: Diffraction processing

Ansgar Philippsen

11:45 am

Tilted Transfer Function (TTF and TCIF): SpotSplitting and deconvolution

Ansgar Philippsen

12:15 pm

Lunch. Segundo Dining Area

1:15 pm

Practical: Image processing of electron diffraction data

-      IPLT: Processing of e-diff pattern (Ansgar Philippsen, Andreas Schenk)

Practical: Image processing trouble shooting session

-      Targeted problem sessions in small groups with requested teachers

Bryant Gipson

Ansgar Philippsen

Andreas Schenk

Henning Stahlberg

Anchi Cheng

4:00 pm

Storer Symposium:     Andreas Engel:

Attempts to observe membrane proteins in their native environment

In: 1309 Surge III

5:30 pm

Dinner at Segundo Dining Area                                                                                            Also:

Storer Reception for invited UC Davis guests
at Putah Creek Lodge.

7:00 pm

SESSION CHAIR: James Evans

DTEM

Nigel Browning

7:45 pm

Low-dose STEM

James Buban

8.30 pm

Open hour

Friday, September 12, 2008

8:00 am

Breakfast.

9:00 am

SESSION CHAIR: Ansgar Philippsen

Merging with the MRC software

-      Phase origin, Defocus, Beam-tilt, Lattice Lines

-      ORIGTILT, MAKETRAN

Anchi Cheng

9:45 am

Merging with the 2dx software

-      Project management

-      Iterative merging and re-processing of images

Henning Stahlberg

10:30 am

Coffee Break

10:45 am

SESSION CHAIR: Tom Walz

3D reconstruction

-      Lattice line interpolation (from e-diff), 3D volume generation, NCS

-      3D Visualization with O / Dino

Andreas Schenk

11:10 am

Hybrid Input Output Algorithm

Bryant Gipson

11:30 am

Calculation of the variance of a 3D map by the bootstrap method

Anchi Cheng

12:00 pm

Lunch.

1:00 pm

Practical: 3D merging of e-cryst data

-      MRC: 3D merging with MRC software (Anchi Cheng, Henning Stahlberg)

-      2dx: management and 3D merging with 2dx_merge (Bryant Gipson, Henning Stahlberg)

Practical: Image processing trouble shooting session

-      Targeted problem sessions in small groups with requested teachers

Bryant Gipson

Ansgar Philippsen

Andreas Schenk

Henning Stahlberg

Anchi Cheng

4:30 pm

Practical: Image processing trouble shooting session

-      Targeted problem sessions in small groups with requested teachers

5:30 pm

Closing Remarks

Ben Hankamer

Tom Walz

Henning Stahlberg

6:00 pm

Free Time

7:00 pm

Dinner:

Hosted by JEOL

Bistro 33

226 F Street

Davis, CA 95616

Tel: (530) 756 4556

http://tinyurl.com/6h5g99

Saturday, September 13, 2008

8:00 am

Breakfast.

9:00 am

Departure