ctffind2
determines defocus and astigmatism for images of arbitrary size (MRC format). Astigmatic angle is measured form x axis (same convetiones as in the MRC 2D image processing programs).
C***************************************************************************** C C CTFFIND2 - determines defocus and astigmatism for images of C arbitrary size (MRC format). Astigmatic angle is measured form C x axis (same convetiones as in the MRC 2D image processing C programs). C C CARD 1: Input file name for image C CARD 2: Output file name to check result C CARD 3: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] C CARD 4: Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep CHENN> C card 5: iNoAstig,dFStartX,dFStartY,dFStartAst CHENN< C C The output image file to check the result of the fitting C shows the filtered average power spectrum of the input C image in one half, and the fitted CTF (squared) in the C other half. The two halfs should agree very well for a C sucessfull fit. C C CS: Spherical aberration coefficient of the objective in mm C HT: Electron beam voltage in kV C AmpCnst: Amount of amplitude contrast (fraction). For ice C images 0.07, for negative stain about 0.15. C XMAG: Magnification of original image C DStep: Pixel size on scanner in microns C Box: Tile size. The program devides the image into square C tiles and calculates the average power spectrum. Tiles C with a significatly higher or lower variance are C excluded; these are parts of the image which are unlikely C to contain useful information (beam edge, film number C etc). IMPORTANT: Box must have a value of power of 2. C ResMin: Low resolution end of data to be fitted. C ResMaX: High resolution end of data to be fitted. C dFMin: Starting defocus value for grid search in Angstrom. C Positive values represent an underfocus. The program C performs a systematic grid search of defocus values C and astigmatism before fitting a CTF to machine C precision. C dFMax: End defocus value for grid search in Angstrom. C FStep: Step width for grid search in Angstrom. CHENN> C iNoAstig: 0=usual operation C 1=searches only defocus, does not vary astigmatism. C dfStart*: Initial defocus values for iNoAstig=1 situation CHENN< C C***************************************************************************** C example command file (UNIX): C C #!/bin/csh -x C # C # ctffind2 C # C time /public/image/bin/ctffind2.exe << eof C image.mrc C power.mrc C 2.6,200.0,0.07,60000.0,28.0 C 128,100.0,15.0,30000.0,90000.0,5000.0 CHENN> C 0,1,1,1 CHENN< C eof C # C C or C C #!/bin/csh -x C # C # ctffind3 C # C time /public/image/bin/ctffind2.exe << eof C image.mrc C power.mrc C 2.6,200.0,0.07,60000.0,28.0 C 128,100.0,15.0,30000.0,90000.0,5000.0 C 1,5780.0,4650.0,27.0 C eof C # C C*****************************************************************************