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MRC file format

by Admin last modified 2008-10-30 08:21

This information is taken from http://ami.scripps.edu/prtl_data/mrc_specification.htm


The MRC file header is of 1024 bytes long, organized as 56 LONG words followed by space for 10 80 byte text labels. The full specification, listed in Table 1, is abstracted from the image2000 Announcement of the MRC Image Processing package by Crowther et al.,  which is currently freely available to academic workers. Use of the programs is described in published papers which are referred to in the publication "Crowther, Henderson and Smith, J. Struct. Biol. (1996) 116, 9-16."

For further information about the MRC file format and MRC Image Processing package which includes routines to read/write MRC image, you may contact Jude Short at jms@mrc-lmb.cam.ac.uk.

Table 1: MRC Image/Map Header Format
LONG Word #Specification
1NX       number of columns (fastest changing in map)
2NY       number of rows   
3NZ       number of sections (slowest changing in map)
4MODE     data type :
     0        image : signed 8-bit bytes range -128 to 127
     1        image : 16-bit halfwords
     2        image : 32-bit reals
     3        transform : complex 16-bit integers
     4        transform : complex 32-bit reals
5NXSTART number of first column in map (Default = 0)
6NYSTART number of first row in map
7NZSTART number of first section in map
8MX       number of intervals along X
9MY       number of intervals along Y
10MZ       number of intervals along Z
11-13CELLA    cell dimensions in angstroms
14-16CELLB    cell angles in degrees
17MAPC     axis corresp to cols (1,2,3 for X,Y,Z)
18MAPR     axis corresp to rows (1,2,3 for X,Y,Z)
19MAPS     axis corresp to sections (1,2,3 for X,Y,Z)
20DMIN     minimum density value
21DMAX     maximum density value
22DMEAN    mean density value
23ISPG     space group number 0 or 1 (default=0)
24NSYMBT   number of bytes used for symmetry data (0 or 80)
25-49EXTRA    extra space used for anything   - 0 by default
50-52ORIGIN   origin in X,Y,Z used for transforms
53MAP      character string 'MAP ' to identify file type
54MACHST   machine stamp
55RMS      rms deviation of map from mean density
56NLABL    number of labels being used
57-256LABEL(20,10) 10 80-character text labels
Symmetry records follow - if any - stored as text as in International Tables, operators separated by * and grouped into 'lines' of 80 characters (ie. symmetry operators do not cross the ends of the 80-character 'lines' and the 'lines' do not terminate in a *). Data records follow.

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